Pharmacokinetic study of dl-tetrahydropalmatine patches by UPLC-MS/MS in rabbits

摘要

#摘要目的：建立延胡索乙素血药浓度的测定方法，考察延胡索乙素贴剂在兔子体内的药代动力学过程。方法：以家兔为实验模型，血药浓度为考察指标，利用UPLC–MS/MS技术测定血药浓度。结果建立了延胡索乙素UPLC–MS/MS测定方法，该方法符合生物样品的分析要求；其兔子体内代谢分布符合一房室模型，Tmax=5.0 h，Cmax=153.12 ng/mL，MRT=10.0h，AUC0-inf=2.10g/mL· h，AUMC=21.15 ug/mL· h2。结论：该测定方法简便、快速、重复性良好，结果准确可靠，灵敏度高，重现性好，可用于延胡索乙素贴剂体内药动学分析研究。%Objective:To establish the measurement method of the plasma concentration and inspect the pharmacokinetic of the dl-tetrahydropalmatine patches. Method:Tandem mass spectrometric detection (UPLC-MS/MS) was employed for the detection of the plasma concentration. Results: We established the mass spectrometry conditions of dl-tetrahydropalmatine, all of which could meet the requirements of analysis of biological samples. The distribution law of dl-tetrahydropalmatine patches accorded with the single compartment model; Tmax=5.0h, Cmax=153.12ng/ml, MRT=10.0h, AUC0-inf=2.10 ug/ml·h, AUMC=21.15ug/ml·h2. Conclusion: The UPLC–MSMS method was useful in the pharmacokinetic study of dl-tetrahydropalmatine patches. It was simple, rapid, accurate and reliable and had high sensitivity and good reproducibility. Consequently, the method could be available for quantitative analysis and pharmacokinetic studies of dl-tetrahydropalmatine patches.