Effect of alternate-day-fasting combined with Lingguizhugan Decoction on blood lipid profiles of hyperlipidemic rats

摘要

Objective:To explore the effects of alternate-day-fasting (ADF) therapy combined with Lingguizhugan Decoction (LD) on blood lipid profiles of hyperlipidemic rats. Methods:Rats were randomly assigned into high-fat-diet (HF) group and normal-diet (ND) group. Hyperlipidemic rats fed with high-fat-diet for 5 weeks were randomly divided into ADF group, alternate-day-fasting with LD (ALG) group and model control (MC) group. The rats in ALG and ADF group were deprived of food for 24 h every other day for 4 weeks. Rats in ALG group were administrated with LD at fasting day. After 4 weeks of ADF therapy, plasma TC, TG, LDL-c and HDL-c were measured in each group. Expression of miR-143 and PPAR-γprotein from adipose was also analyzed. Results:When compared with MC group, after 4 weeks of ADF or combined ADF and LD therapy, the body weight was evidently reduced in ADF and ALG groups (P=0.028, P=0.036 by wk 8). The levels of plasma TC and TG decreased in ADF group and ALG group, which were significantly lower than those in MC group (P<0.001, P=0.045;P<0.001, P=0.005). However, the body weight and level of TC and TG in ALG group showed non-statistical difference in comparison with ADF group (ALG vs. ADF, P >0.05). Expression of miR-143 and PPAR-γ were higher in MC group than that in NC group (P < 0.001). Compared with MC group, expression of miR-143 and PPAR-γwere significantly decreased in ADF (P=0.038, P=0.015) and ALG (P=0.007, P<0.001) groups. When compared with ADF group, expression of miR-143 and PPAR-γwere significantly decreased in ALG (P = 0.041, P = 0.046) group. Conclusion:ADF therapy alone not only reduced blood lipids, but also inhibited miR-143 and PPAR-γ protein expression in visceral adipose tissue. However, LD couldn't reduce the levels of blood lipid profiles more effectively than using ADF alone. Perhaps the effects of LD combined with ADF in the prevention of hyperlipidemia need further exploration.%目的:观察隔日禁食疗法联合苓桂术甘汤对高脂血症大鼠血脂和脂肪组织miR-143及PPAR-γ表达的影响.方法:52只Wistar大鼠随机分成高脂饲料组和普通饲料组,饲养5周,造模成功后,高脂饲料组随机分成模型对照组(n=8)、隔日禁食组(n=8)和中药禁食组(n=8),隔日禁食组与中药禁食组均行隔日禁食24小时,中药禁食组大鼠在禁食日以苓桂术甘汤灌胃,连续4周.每周称重和计算食量.造模4周后大鼠处死,测定血总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c),以及大网膜脂肪组织中miR-143和PPAR-γ的表达.结果:隔日禁食疗法干预4周后,与模型对照组相比,隔日禁食组与中药禁食组体体重下降(P=0.028,P=0.036 by wk 8),隔日禁食组与中药禁食组TC、TG水平均下降(P<0.001,P=0.045;P<0.001,P=0.005).但是与隔日禁食组相比,中药禁食组TC、TG水平没有显著性降低(P>0.05),两组间的大鼠体重无显著差异(P>0.05).与空白对照组相比,模型对照组miR-143和PPAR-γ表达明显增加(P<0.001),与模型对照组相比,隔日禁食组大网膜脂肪miR-143和PPAR-γ表达均下降(P=0.038,P=0.015),中药禁食组miR-143和PPAR-γ表达显著下降(P=0.007,P<0.001).与隔日禁食组相比,中药禁食组能够促进miR-143和PPAR-γ表达显著下降(P=0.041,P=0.046).结论:隔日禁食疗法能够改善高脂血症大鼠模型的血脂水平和抑制脂肪组织miR-143和PPAR-γ的表达,联合苓桂术甘汤后对血脂水平的改善作用却未见明显增强.苓桂术甘汤联合隔日禁食疗法对高脂血症的防治作用还需进一步研究.