Molecular mechanism of Mimenghua Granules in treating dry eye

摘要

Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingredients and action target of Mimenghua Granules through Traditional Chinese Medicine Systems Pharmacology database and analysis platform;mining dry eye-related diseases through disease gene database,gene target;use the functional protein combined network database STRING to draw the component-target and disease-target PPI networks,and extract the intersection of these two networks;use DAVID database analysis to screen key targets and analyze the mechanism of action.Results:A total of 593 active ingredients related to Mimenghua Granules were retrieved from the Traditional Chinese Medicine Systems Pharmacology database,and 59 blood active ingredients were obtained by screening based on pharmacokinetic parameters,and 680 targets related to these ingredients were retrieved;from disease genes,the database searches for 47 genes directly related to dry eye;3 key genes(ICAM1,IFNG,and IL-6)were obtained after the intersection of the component target and disease target PPI network;these genes are mainly involved in natural killer cell-mediated cytotoxicity,Jak-STAT signaling pathway,and Cytokine-cytokine receptor interaction.Conclusion:The mechanism of Mimenghua Granules in treating dry eye is related to the interaction of cytotoxic pathway,Jak-STAT signal pathway,and cytokines.The key gene targets are ICAM1,IFNG,and IL-6.