首页> 中文期刊> 《作物学报:英文版》 >ALM1, encoding a Fe-superoxide dismutase, is critical for rice chloroplast biogenesis and drought stress response

ALM1, encoding a Fe-superoxide dismutase, is critical for rice chloroplast biogenesis and drought stress response

     

摘要

Chloroplasts are the center of plant life activities including photosynthesis,growth and development,and abiotic stress response.Chloroplast development and biogenesis in rice have been studied in detail,but how does abiotic stress affect chloroplasts is less studied.We obtained an albino mutant,alm1,whose chlorophyll content was greatly decreased.Transmission electron microscopy showed that chloroplast development in alm1 was blocked,especially in thylakoid-like structures,which could not form normally.The ALM1 gene encodes a chloroplast-localized superoxide dismutase.Full-length ALM1 successfully restored the non-albino phenotype,and in knockout lines,the albino phenotype reappeared.The ALM1gene is expressed mainly in young leaves.alm1 plants died as a consequence of excessive reactive oxygen accumulation after the third-leaf stage.A series of biochemical assays verified that ALM1 interacted with the OsTrxz protein,which is one of the components of plastid-encoded RNA polymerase (PEP) complexes.A western blot experiment indicated that ALM1 played an important role in stabilizing OsTrxz in rice.An overexpression test of ALM1 revealed that ALM1 can increase drought resistance by removing excess reactive oxygen in rice seedlings.This study suggests that ALM1 not only participates in rice chloroplast biogenesis,but also increases rice stress resistance by scavenging excess reactive oxygen.

著录项

  • 来源
    《作物学报:英文版》 |2021年第5期|P.1018-1029|共12页
  • 作者单位

    Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China;

    Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China;

    College of Bioscience and Bioengineering Hebei University of Science and Technology Shijiazhuang 050000 Hebei China;

    Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China;

    Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 禾谷类作物;
  • 关键词

    Rice; Chloroplast biogenesis; PEP complex; Drought stress;

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