Allograft of developing metanephroi into omenta of cyclosporine-treated adult rats after preservation

摘要

To evaluate the animal models and the effect on organogenesis and function of embryonic metanephroi allografted into cyclosporine-treated aduld rats after their preservation in histidine-tryptophan-ketoglutarate (HTK) solution.Methods Whole metanephroi preserved in HTK for 3 days from embryonic day 16 and 17 (E16,E17) of Sprague-Dawley (SD) rats were grouped and allografted into the omenta of cyclosporine-treated SD adult rats which had one kidney resected.E16 metanephroi implanted directly into omenta were used as controls.3 to 4 weeks after implantation,metanephroi allografted in host rats were removed for histopathological and electronic microscopy (EM) examination or anastomosed for renal function measurement 8 weeks later.Results 3 to 4 weeks post-implantation,E16 and E17 metanephroi had formed mature nephrons and collecting ducts with few lympocytic infiltration.EM examination showed they had cellular ultrastructure of normal nephrons and collecting ducts.E16 and E17 metanephroi allografted after preservation for 3 days had no significantly different values of wet weight (P=0.346,P=0.705),urine volume (P=0.396,P=0.687),and creatinine clearances (P=0.469,P=0.543) from those of E16 metanephroi implanted directly.Conclusion E16 and E17 metanephroi allografted into cyclosporine-treated adult rats undergo growth and differentiation and exhibit excretory function after preservation in HTK solution for 3 days.9 refs,1 tabs.