Sodium nitroprusside preventing pulmonary ischemia-reperfusion injury

摘要

To study the role of sodium nitroprusside in preventing pulmonary ischemia-reperfusion injury and the possible mechanism.Methods Fourteen pigs were randomly divided into 2 groups:control group underwent controlled reperfusion with reperfusion solution (leukocyte-depleting blood:modified buckberg perfusate=4∶1) for 10 min at lower pressure of 18 mm Hg and 37 ℃±1 ℃ before removing the pulmonary artery clamp;experimental group were subjected to controlled reperfusion with reperfusion solution before removing the pulmonary artery clamp,followed by infusion of sodium nitroprusside (10 μg·kg -1 ·min -1 for 10 min) via pulmonary artery.Blood oxygen pressure,pulmonary vascular resistance,pulmonary compliance and the changes in pulmonary oxygenation function were assessed 0.5,1 and 2 h later.After the trail,the contents of water,NO and MDA in pulmonary tissues were measured.Results In the experimental group,left lung oxygenation function and pulmonary compliance was significantly better (P<0.01),pulmonary vascular resistnace,the contents of MDA and water were lower than in the control group.The content of NO in experimental group (114.41±6.77)μmol/L was significantly increased as compared with that in control group (56.67±7.42)μmol/L,(P<0.01).Conclusion Sodium nitroprusside can significantly attenuate pulmonary ischemia-reperfusion injury by significantly increasing the level of NO in lung tissue.5 refs,1 tab.