Objective To observe the morphological changes and myoblast proliferation dynamics of skeletal muscle after chronic spinal cord compression in rats. Methods A total of 50 Wistar rats were randomly divided into normal group ( n = 10) ,sham operation group (Group A, n = 10) and chronic compression group (Group B, n = 30). The rats in the Group B were given gradual compression on the posterior spinal cord using blunt platic screw. Compression degree reached 20% (n = 10),40% (n = 10)and60% (n = 10) respectively after two months. Following sacrifice of rats, gastrocnemius muscle was removed for HE staining and preparation of cell suspension. HE staining and image analysis system were used to measure the diameter and cross section area of rat gastrocnemius muscle cells and cytoflowmeter to detect the periodical changes of myoblast. Results Atrophy and degeneration of gastrocnemius muscle cell occurred in every chronic compression group. The diameter and the cross section area of rat were decreased with
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