首页> 中文期刊> 《亚热带植物科学》 >珍贵用材树种红椿的组培育苗技术初探

珍贵用材树种红椿的组培育苗技术初探

         

摘要

The seeds of Toona ciliata were used as explants to study the tissue culture of T. ciliata. The results showed that the MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1 media was good for the bud induction of the asepsis seedling, the optimal bud proliferation culture medium was 2/3MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1+GA3 1.0 mg·L-1, and the optimal root induction culture medium was 1/2MS+IBA 0.5 mg·L-1, The plantlet was transplanted with the matrix of the peat soil, yellow subsoil and river sand, and the survival rates up to 85%.%以红椿种子为外植体材料,对其组织培养技术进行初步研究。结果表明,适宜红椿种子无菌苗芽诱导的培养基为MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1,适宜的芽继代增殖培养基为2/3MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1+GA 31.0 mg·L-1,适宜生根培养基为1/2MS+IBA 0.5 mg·L-1,以泥炭土、黄心土、河沙按1∶1∶1混合作为基质,移栽成活率达85%以上。

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