丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应

摘要

To explore the influence of arbuscular mycorrhizal fungus (AMF), P level and auxin on plant lateral root (LR) formation, AMF strain (Rhizophagus irregularis BGC JX04B) was inoculated to Poncirus trifoliata seedlings at two P levels, which was applied with IBA, TIBA and water as control, and the regulative effects of AMF, P level and auxin on LR formation of trifoliate orange were investigated. The results showed that AMF did not significantly affect the plant biomass and LR number, but significantly decreased the 1st order LR length. P level did not significantly affect the plant biomass, LR number and length. TIBA significantly decreased the plant biomass, LR number and length, while IBA did not significantly influence these parameters. AMF and auxin showed significant interaction on the primary root length, and AMF, P level and auxin showed significant interaction on the 2nd and 3rd order LR number. These data suggested that auxin signaling pathway could be involved in the regulation of AMF and P level on LR formation, and the auxin transportation should be the key factor in LR formation of trifoliate orange.%为探讨丛枝菌根真菌(AMF)、磷水平和生长素对植物侧根形成的影响，在两种磷水平下接种AMF(Rhizophagus irregularis BGC JX04B)，施用 IBA、生长素运输抑制剂(TIBA)，观察 AMF、磷水平和生长素对枳 Poncirus trifoliata 幼苗侧根形成的调控效应。结果表明，AMF 对植株生物量及各级侧根数量无显著影响，但显著降低一级侧根长度；磷水平对植株生物量、侧根数量及长度无显著影响；TIBA 显著降低植株生物量、侧根数量和侧根长度，而 IBA 对各项指标无显著影响。AMF 和生长素对主根长度的影响存在显著互作；AMF、磷水平和生长素对二级和三级侧根数量的影响存在显著互作。因此，AMF 对枳侧根形成的调控可能涉及生长素信号途径，而生长素运输是枳侧根形成的关键因素。