量子点(QD)具有抗漂白能力强、量子产率高、激发谱宽和发射谱窄等优点,在生物荧光标记领域有较广泛的应用.利用QD 585和Hoechst 33342 (H342)双标记小鼠卵母细胞中DNA甲基转移酶(Dnmt)和染色体,通过双光子成像同时双通道探测它们的荧光图像,获得了Dnmt蛋白表达的三维空间分布.发现老龄小鼠卵母细胞不适合体外培养成熟:该成熟方式不仅引起老龄小鼠卵母细胞Dnmt蛋白含量的变化,而且还改变了它们在胞质中的空间分布.这些改变可能与异常的甲基化修饰有关.%Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors' results showed that aging mouse oocytes may be not suitable for in-vitro maturation- both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA meth-ylation modification.
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