新育三系杂交稻亲本的稻瘟病抗性基因分子标记检测研究

摘要

[Objective] Identification on rice blast resistance genes rice breeding parents would contribute to understand the distribution of resistance genes,which can guide rice disease resistant varieties breeding and cultivating.[Method] The new breeding 32 hybrid rice parents were detected by using the molecular markers closely linked to the rice blast resistance genes pi2,pikm and pita,and identity of blast resistance in the fields.[Result] 2 of them contained the resistance gene pi2,pikm and pita,12 of them contained the resistance gene pi2 and pita,2 of them contained the resistance gene pikm and pita,only 1 of them contained the resistance gene pita,and 3 of them resistance to blast by field identity.[Conclusion] New breeding three-lines sterile line De 5A and restore-line luhui 37 can be applied in backbone parents for breeding resistant varieties.%[目的]了解不同抗病基因在育种亲本材料上的分布,为选育抗病品种提供参考.[方法]利用与抗稻瘟病基因ppi2、pikm和pita紧密连锁的分子标记检测分析新育成的杂交水稻亲本材料32份,同时进行田间抗性鉴定.[结果]含有pi2、pikm和pita的有2份材料;含有pi2和pita的有12份材料;含有pikm和pita的有2份材料;含有pita的有1份材料.田间鉴定抗稻瘟病的有34份材料.[结论]新育三系不育系德5A和恢复系泸恢37可作为抗病品种选育的骨干亲本应用.