组合外源添加剂对杂交狼尾草厌氧发酵产沼气的影响

摘要

[Objective] The present research aimed to study the effect of combined exogenous additives on biogas production of anaerobic fermentation of hybrid pennisetum.[Method] A simulation experiment was conducted for 35 days at 35 ℃,with mass proportion of total solid (TS) being 6 ％,the rate of vaccination being 20 ％ and the initial pH value being 7.0.The combinations of six additives were established by orthogonal design.[Result]The effect of the 6 additives decreased in the following order as cellulose enzyme ＞ EDTA-2Na ＞ tween 20 ＞ CoCl2 ＞ NiCl2 ＞ FeCl2.And the optimized combination was decided as follows,10.0 mg/L FeCl2,1.0 mg/L CoCl2,1.0 mg/L NiCl2,15 g/L cellulase,250 mg/L tween 20,and 0 g/L EDTA-2Na,and the accumulated biogas yield increased by 29.52 ％ than that of control treatment.[Conclusion] The exogenous additives could efficiently promote the activity of methanogens and increase biogas yield of anaerobic fermentation of hybrid pennisetum.%[目的]为了研究组合外源添加剂对杂交狼尾草厌氧发酵产沼气的影响.[方法]采用正交试验设计添加剂组合方式,在中温35℃、初始pH值为7.0、发酵体系TS质量分数为6％、VS接种率为20％条件下进行序批次厌氧发酵试验.[结果]外源添加剂影响力顺序为:纤维素酶＞ EDTA-2Na＞吐温20＞CoCl2＞NiCl2＞FeCl2.优选的添加剂组合方案为:FeCl2 10.0 mg/L,CoCl2 1.0 mg/L,NiCl2 1.0 mg/L,纤维素酶15 g/L,吐温20 250 mg/L,EDTA-2Na 0 g/L,该条件下累积产气量较对照组提高29.52％.[结论]组合外源添加剂能够明显影响杂交狼尾草厌氧发酵过程,并能够有效改善甲烷菌活性,提高原料产气效率.