急性操作胁迫对四指马鲅幼鱼肝脏组织结构和氧化应激的影响

摘要

To understand the effects of acute handling stress on liver tissue and oxide stress of juvenile Eleutheronema tetradactylum,we sampled at different time after handling stress out of water and then observed the change of liver tissue and detected the activities of antioxidant enzymes.The results show that with prolongation of time of water stress,the degree of liver tissue injury appeared a trend of rising and then decreasing,and the basic structure of liver was similar with that of the control group at 24th hour.The SOD and CAT had the same changing trend,i.e.,increased at 2nd hour significantly after the stress (P ＜ 0.05),reaching the maximum value,and then began to drop until they were significantly lower than those in the control group (P ＜ 0.05).The MDA content increased at first and then decreased at 2nd ～ 12th hour during the stress,and increased after 12th hour.The GSH content had no significant change in the first two hours (P ＞ 0.05),followed by a decreasing-increasing trend,but its content was still lower than that in the control group significantly (P ＜ O.05).During the first two hours of acute handling stress,the T-AOC activity increased significantly (P ＜ 0.05),then decreased gradually,significantly lower than that of the control group (P ＜ 0.05).It is concluded that the handling stress out of water did have some effects on the liver tissue and antioxidant enzymes of E.tetradactylum,but with extension of stress time,the tissue structure and antioxidant activity had certain recovery.%为探究急性操作胁迫对四指马鲅(Eleutheronema tetradactylum)幼鱼肝脏组织结构以及氧化应激的影响,该研究在离水操作胁迫后不同时间取样,观察肝脏显微结构的变化,并检测相关抗氧化酶活性.结果显示,随着离水胁迫时间的延长,肝脏组织损伤程度呈现先上升后下降的趋势,胁迫第24小时肝脏基本结构与对照组相似.超氧化物歧化酶(SOD)和过氧化氢酶(CAT)有相同的变化趋势,胁迫后第2小时显著增加(P＜0.05)并达到最高值,然后开始下降,最后其值显著低于对照组(P＜0.05);丙二醛(MDA)含量在胁迫第2～第12小时呈现先升高后下降的变化趋势,第12小时后又有所上升;还原型谷胱甘肽(GSH)含量胁迫2h没有显著变化(P＞0.05),之后呈先下降后上升的趋势,但其含量仍显著低于对照组(P＜0.05);总抗氧化能力(T-AOC)胁迫第2小时显著上升(P＜0.05),之后下降,实验结束时显著低于对照组(P＜0.05).结果表明离水操作胁迫对四指马鲅幼鱼肝脏组织结构以及抗氧化酶产生一定的影响,但随着胁迫时间的延长,其组织结构以及抗氧化能力都有一定的恢复趋势.