Soil enzyme activities and soil fertility were studied to explore the long-term fertilization on soil enzyme activities and its relationship with soil fertility on the basis of 29-years long-term site-specific experiment with different fertilization methods. The results showed that combined application of chemical fertilizers and organic manure could significantly increase the soil organic matter, total nitrogen, total phosphorus, alkali-soluble nitrogen, available phosphorus content and enhance the soil urease, phosphatase, invertase, catalase, dehydrogenase activity. The results of correlation analysis showed that soil urease, phosphatase, invertase catalase and dehydrogenase activity positively corrected with organic matter, total nitrogen, total phosphorus and alkali-soluble nitrogen significantly, and the integrated activities of soil urease, phosphatase, invertase, catalase dehydrogenase activity could reflect the changing trend of black thin-layer soil quality in the farmland after long-term fertilization.%利用29年的长期定位试验,对不同的施肥方式下薄层黑土酶活性及肥力进行了研究,探讨长期施肥对土壤酶活性及与土壤肥力的影响.结果表明,化肥与有机肥配合施用能明显提高土壤有机质、全氮、全磷、碱解氮、有效磷含量,增强土壤脲酶、磷酸酶、转化酶、过氧化氢酶、脱氢酶活性.相关分析结果显示,土壤脲酶、磷酸酶、转化酶、过氧化氢酶、脱氢酶活性与有机质、全氮、全磷、有效磷、碱解氮呈显著正相关.土壤脲酶、磷酸酶、转化酶、过氧化氢酶、脱氢酶综合活性可以反映长期施肥后薄层黑土质量的变化趋势.
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