多层螺旋CT同层动态扫描结合MPR技术诊断肝外胆管癌的研究

摘要

Objective:To investigate the cell cycle changes of hepatoma cells and the rote of antisense oligonucleotide targeting bFGF.Methods:Inhibition of bFGF protein expression was investigated by conical microscopy analysis and Western blot in the best condition of transfecting antisense oligonucleotide targeting bFGF.Cell cycle and apoptosis were detected with flow cytometry analysis.Results:Treatmenl with antisense oligonucleotide of bFGF not only reduced the expression of bFGF by conical microscopy and Western blot analysises,but also increased the apoptosis of HepG2 cells(P＜0.01).Conclusion:bFGF may take part in apoptosis regulation of hepatoma cells and be used as a target of hepatocel-lular carcinoma therapy.