表达HPV16L1、L2和E7蛋白的非复制重组痘苗病毒的构建

摘要

Objective:To construct a non-replicating vaccinia virus expressing human papitlomavirus 16 (HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer. Methods:Using vaccinia virus vector, we generated a strain of non-repli-cating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western-bloting. Results:We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay. Conclu-sion:NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.