白细胞介素-10对风湿性心脏病患者外周血单个核细胞NF-ΚB/p65表达的影响

摘要

Objective To investigate the expression of nuclear factor kB/p65 on peripheral blood mononuclear cells ( PBMCs) in Rheumatic heart disease (RHD) patients and effect of Interleukin-10 (IL-10) and the relation with cardiac function.Methods All patients undertook ultrasonic cardiography. PBMCs isolated from peripheral blood in RHD patients ( n= 22 ) and healthy subject( n= 15)were divided into 3 groups:control group, lipopolysaccharide(LPS) stimulate group and IL-10 group, The expression of NF-κB/P65in PBMCs were measured by immunocytochemical staining. Results The expression of NF-κB/P65 is increasing with heart function failure in RHD patients. Compared with the healthy subjects,the expression of NF-κB/P65 on PBMCs in RHD patients was found significantly increased [ ( 24.8 ± 8.6 ) ％ 比 ( 8.2 ± 3.4 ) ％, P ＜ 0. 01]. It is increasing with heart function failure in RHD patients. In LPS stimulation, the expression of NF-κB/P65 on PBMCs in RHD patients and healthy subject was found significontly increased( P ＜0. 01 ). IL-10 significantly inhibited this increment in RHD patients and healthy subject (P ＜0. 01 ). Conclusion The expression of NF - kB/P65 in RHD patients is found significantly increased, but IL-10 can inhibit LPS stimulating increment of NF-κB/P65. It may be a predictor of the severity of heart remodeling and heart function failure.%目的 探讨白细胞介素-10(IL-10)对风温性心脏病患者(RHD)外周血单个核细胞(PBMCs)核转录因子appaB(NF-κB)/p65表达的干预作用及NF-κB与心功能的关系.方法 22例RHD患者和15名健康对照者进行心脏超声心动图检查,并对所有入选者分离出的PBMCs进行培养,并将培养的PBMCs分成3组:空白对照组、脂多糖(LPS)组和白细胞介素-10组.对3组培养后的PBMCs NF-κB/p65的表达进行免疫组化染色检测和半定量分析.结果 RHD患者PBMCs NF-κB/p65胞核染色阳性率明显高于健康对照者[(24.8 ±8.6)%比(8.2±3.4)%,P＜0.01];RHD患者随着心功能恶化NF-κB/N5表达水平逐渐升高;脂多糖组RHD患者和健康对照者PBMCs NF-κB/p65胞核染色阳性率与空白对照组比较均显著增加(P＜0.01);白细胞介素-10组RHD患者和健康对照者PBMCs NF-κB/p65胞核染色阳性率与脂多糖组比较则显著下降(P＜0.01).结论 RHD患者NF-κB/p65表达增高,而白细胞介素-10可下调脂多糖刺激下NF-κB/p65的表达增高;它的检测有助于对RHD患者心功能恶化的判断.