血清微小RNA可能是急性心肌梗死的标志物

摘要

目的 观察急性心肌梗死患者血清中微小RNA (miRNA)水平的变化.方法 采用3’端加多聚poly(A)尾的反转录后荧光定量PCR的方法测定17例急性心肌梗死患者和11例健康对照者血清中miR-1的水平,并测定其血清肌酸激酶(CK)、肌酸激酶同工酶MB(CK-MB)及心肌肌钙蛋白T(cTnT)水平.结果 心梗组miR-1水平较对照组升高(P＜0.01)；心梗组患者血清miR-1较对照组均值升高的倍数与其血清CK-MB较对照组均值升高的倍数存在正相关关系(r =0.52,P＜0.05).结论 miR-1有可能作为急性心肌梗死的标志物,miR-1水平升高的机制可能与心肌梗死灶周围的缺血组织释放增加有关.%Objective To evaluate the potential application of serum microRNA-1 (miR-1) as a diagnostic marker in acute myocardial infarction (AMI). Methods The serum levels of miR-1 in 17 AMI patients and 11 health control subjects were determined by using the method of poly (A) tailing-based SYBR quantitative PCR, The serum levels of creatine kinase (CK) , creatine kinase-MB (CK-MB) and cardiac troponin t (cTnT) were also determined. Results The level of serum miR-1 was significantly increased in patients with AMI; The multiple of miR-1 level in AMI group/mean value of control group had a positive correlation with the multiple of serum CK-MB level in AMI group/mean value control group. Conclusions The results suggest that serum miR-1 could be a potential diagnostic biomarker for AMI. The mechanism of elevated miR-1 may be associated with increased release of ischemic tissues around the infarct.