Nogo-A蛋白在大鼠心肌细胞中的表达变化及其在细胞缺氧性凋亡中的作用

摘要

Objective To investigate the expression of Nogo-A protein in rat cardiomyocytes and its effect on hypoxiainduced apoptosis.Methods ① Nogo-A protein expression was determined in adult rat cardiomyocytes by using immunohistochemistry and Western blot.② The acutely isolated myocardial cells were divided into three groups:the control group,model group and intervention group.To establish the hypoxia-induced myocardial cell apoptosis models in the model group and intervention group,the intervention group was treated with the anti-Nogo-A antibodies before the modeling.The apoptosis,the expre.ssion of Nogo-A protein,Caspase-3 and Caspase-12 in three groups were observed.Resets ① Adult cardiomyocytes:the expression of Nogo-A protein was positive.② Cardiomyocytes under hypoxia:the expression of NogoA protein was positive; the intervention group and the model group appeared apoptotic cells,but the intervention group was significantly less than the model group; the positive rates of Caspase-3 and Caspase-12 expression were significantly higher in the intervention group and model group than those in the control group,but the intervention group was significantly lower than the model group,P ＜ 0.05.Conclusions There was a large amount of expressed Nogo-A protein in myocardial cells.Nogo-A protein may be involved in the hypoxia-induced apoptosis of myocardial cells.%目的 探讨Nogo-A蛋白在心肌细胞中的表达及其在细胞缺氧性凋亡中的作用.方法 ①采用免疫组化法和免疫印记法测定成年大鼠心肌细胞(成熟心肌细胞)Nogo-A蛋白表达.②急性分离大鼠心肌细胞分为空白组、模型组及干预组,模型组及干预组建立心肌细胞缺氧模型,其中干预组制模前采用抗Nogo-A抗体干预.观察三组细胞凋亡情况、Nogo-A蛋白及Caspase-3和Caspase-12表达情况.结果 ①成熟心肌细胞:Nogo-A蛋白表达呈阳性.②缺氧心肌细胞:Nogo-A蛋白表达呈阳性；干预组及模型组均出现凋亡细胞,但干预组明显少于模型组；干预组及模型组Caspase-3和Caspase-12表达阳性率均明显高于空白组,但干预组明显低于模型组,P＜0.05.结论 心肌细胞中存在大量表达的Nogo-A蛋白；Nogo-A蛋白可能参与了心肌细胞的缺氧性凋亡.