生牛乳中金黄色葡萄球菌的耐药性研究

摘要

In order to investigate the drug resistance of Staphylococcus aureus in raw cow milk,130 sam-ples of raw milk from 6 different areas of Shandong Province were collected for the isolation,identification and phenotypic analysis of S.aureus.The results showed that S.aureus were detected in 57 samples,and the av-erage detection rate was 43.85%.The susceptibility test of 57 samples to 12 kinds of antibiotics belonged to 8 classes showed that the resistance rate to penicillin was the highest of 87.72%,and the resistance rates to am-picillin,erythromycin and clindamycin reached higher level,which were 84.21%,78.95%and 56.14%re-spectively.In addition,50 strains showed multiple drug resistance,and the strains resistant to three and five drugs were the most,and the drug multi-resistant patterns were various and different from areas.The results could provide references for the standard use of antibiotics in dairy industry in Shandong Province.%为调查山东省生牛乳中金黄色葡萄球菌的耐药性情况,本研究采集山东省6个不同地区的130份生牛乳样品,进行金黄色葡萄球菌的分离、鉴定及耐药表型分析.结果表明,共有57份样品判定检出金黄色葡萄球菌,平均检出率为43.85%.对57份样品进行8大类12种抗生素的药敏试验,结果表明对青霉素的耐药率最高,为87.72%,同时对氨苄西林、红霉素和克林霉素的耐药率也达到较高水平,分别为84.21%、78.95%和56.14%.另外,57株金黄色葡萄球菌中有50株为多重耐药菌株,其中以耐3种药物与耐5种药物的菌株数最多,且耐药谱种类较多,受地区差异影响较大.该结果可为山东地区奶牛业规范使用抗生素提供一定参考.