The twin-embryos seed of autotetraploid rice DER 10-04-01 was taken as the indues material, and analyzed initiation rate, average callus index and growth rate . The effect of different concentration and ratio of 2,4-D,NAA and NOA on callus induction rate, growth rate and the average callus index so as to the most suitable tetraploid double embryo seedlings of DER 10-04-01 rice was analysed. Experiments showed that; rate, average, callus index and growth rate were all effected by 2,4-D,NAA and NOA ,and the best combination for the tetraploid double embryo seedlings DER 10-04-01 rice ( MS + 2,4-D 2 mg/L + NAA 2 mg/L + NOA 3 mg/L) could guide cultures for clone induction .%以同源四倍体双胚苗水稻DER 10-04-01成熟种子为试验材料进行无性系诱导,统计愈伤组织的诱导率、平均愈伤指数和愈伤组织的生长率.运用田口设计方法分析培养基中2,4-D,NAA和NOA不同的浓度配比对愈伤组织诱导率、生长率和平均愈伤指数的影响,由此获得最适于试验材料愈伤组织诱导和培养的优化组合.试验结果表明,2,4-D,NAA和NOA均对愈伤组织的诱导率、平均愈伤指数和生长率有一定的影响,其最优培养组合(MS +2,4-D 2 mg/L+ NAA2 mg/L+ NOA 3 mg/L)在无性系诱导和培养中有一定的参考价值.
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