茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用

摘要

[目的]研究茉莉酸合成相关基因Spr2与LePrs对番茄抗根结线虫的影响,探讨茉莉酸在番茄抗根结线虫病中的作用.[方法]以番茄茉莉酸合成突变体spr2、茉莉酸过量表达转基因番茄35S(::)PS及野生型CM为试材,研究外源喷施茉莉酸甲酯及不同番茄试材相互嫁接对接种根结线虫后番茄根结数、蛋白质酶抑制剂PI-II表达及Spr2、LePrs基因转录水平的影响.[结果]番茄茉莉酸合成突变体spr2较野生型与茉莉酸过量表达转基因番茄35S::PS容易感染根结线虫,外源喷施茉莉酸甲酯降低了番茄根结数.以茉莉酸过量表达转基因番茄35S(::)PS为砧木,可以提高嫁接植株对根结线虫的抵抗能力.[结论]Spr2突变降低了番茄对根结线虫的抗性, LePrs过量表达可以提高植株对根结线虫的抗性.因此,茉莉酸在番茄抗根结线虫病中起到了一定的作用.%[Objective] Spr2 and LePrs were related with JA synthesis, the effects of the two genes on the response of tomato to root-knot nematodes were studied. [Method] In this paper, JA biosynthetic mutant (spr2 plants) and JA-overexpression transgenic plants (35S::PS plants) of tomato as well as wild tomato species (CM) were used, and the effects of MeJA sprays and grafting on root-knot numbers, P1-II content and Spr2, LePrs transcription levels, before and after inoculating root-knot nematodes, were analyzed. [Result] Compared with CM and 35S::PS plants, spr2 plants was more susceptible to root-knot nematodes. Spraying MeJA on tomato leaves could reduce root-knot numbers. Using 35S::PS plants as stock improved grafting tomato resistance to root-knot nematodes. [Conclusion] Resistance to nematodes in JA biosynthetic mutant (with mutant Spr2) was reduced, while in JA-overexpression transgenic tomato (with over-expressing LePrs) it was increased. So Jasmonic acid played a role in the response of tomato to root-knot nematodes.