A rice heading-date-related mutant was isolated from a 60 Co-γ-ray-induced mutation pool of Zhejing 22,a conventional japonica cultivar in Zhejiang Province,China.The mutant was characterized by a delayed heading date of almost 20 d longer than the wild type plant.Genetic analysis revealed that the mutation was controlled by a single nuclear-encoded recessive gene that was designed as HD(t)(heading date tentatively).To isolate the HD(t) gene,a map-based cloning approach was employed using 479 F 2 mutant individual plants derived from the cross between the hd(t) mutant(japonica) × Zhenshan 97(indica).Finally,the HD(t) gene was mapped to an approximate 53 kb region between the insertion and deletion(InDel) markers of 10-61W and 10-66W on chromosome 10.According to the genome sequence of Nipponbare,the target region contains 11 annotated genes.It is helpful for future cloning of HD(t) gene based on this fine mapping results.
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