流式细胞术在外用避孕药精子功能评估中的应用

摘要

流式细胞术(flow cytometry,FCM)是一种单细胞快速定量分析技术,为精子功能研究提供了一种高通量、多参数的检测手段.FCM在外用避孕药对精子功能评估中的应用,主要包括精子质膜完整性、DNA完整性、顶体反应、线粒体功能、氧化应激和精子凋亡等方面.表明FCM是精子功能研究的有效工具,有助于阐明新型外用避孕药的药效和作用机制.%Flow cytometry (FCM) is a quantitative technology employed in cell counting, sorting, biomarker detection and protein engineering. It allows simultaneous multiparametric analysis of the sperm function. In this review we introduced the application of FCM in the evaluation of topical contraceptives, including plasma membrane integrity, DNA intactness, changes induced during capacitation, mitochondrial status, oxidative stress as well as apoptotic-like changes. It is concluded that FCM is an important tool in the research of sperm function, which helps to clarify the effect and mechanism of novel topical contraceptives.