To study the functional changes of immunological surveillance after vasectomy, we have dynamically observed the influence of vasectomy on the functions of T lymphocytes and macrophages of peripheral blood from vasectomized animals as the experimental model. The results showed that: (1)PHA and Con-A hlduced mitogenesis of T lymphocytes from the rabbits vaseetomized for eight months was significantly inhibited (P<0.01) and recovered in rabbits vaseetomlzed for 12, 18 and 22 months respectively; (2)The IL- 2 secretion from peripheral blood mononuelear cells (PBMCs) in the rabbits vaseetomized for 8 months was reduced remarkably (P<0.01) and reached the normal level in the rabbits vasectomized for 12 months; (3) A significant correlation was found between Con-A induced mltogenesis of T lymphoeytes and the IL-2 secreted from PBMCs (r=0.661, P<0.01); (4) The IL-1 secretion from peripheral blood macrophages induced by LPS significantly increased in the rabbits vaseetomized for 8 months (P<0.02) and returned to the normal level in the rabbits vasectomized for 12, 18 and 22 months respectively; (5) The level of plasma lysozyme increased significantly in the rabbits vaseetomized for 8 months (P < 0.05) and returned to the normal in the rabbits vaseetomized for 12 months; (6) The level of IL-1 secreted by the macrophages and the level of plasma lysozyme were closely correlated (r=0.576, P<0.001). The present results may provide an experimental evidence for the evaluating the functional changes of immunological surveillance in vasectomized animals.
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