A Simple Method of Detecting Chlamydia Trachomatis Using Enzymatically Amplified DNA and Immobilized Probes on Microtiter Plate

摘要

We have developed a simple and economical method for Chlamydia trachomatis detecting, called microtiter plate hybridization (PCR-MPtt) , which may replace standard PCR. This method is similar to that of an ELISA. Briefly, the PCR products labeled at the 5' termini with biotin were hybridized with probes immobilized on a microtiter well