The pathogenesis of premature ovarian failure(POF)remains unknown.Accu-mulated evidences indicate that abnormality in the immnune system may be one of themajor reasons and the imbalance of T help type Ⅰ(Th1)and T help typeⅡ(Th2)may play an important role in the process.Study of the distribution and functional status of Th1/Th2 may be helpful for evaluating the pathogenesis of POF.13 patients with id-iopathic POF and 7 women of reproductive age with normal cycle were enrolled in this study.The percentage of Th1 and Th2 cells from peripheral blood of the patients and the controls was studied using FITC labelled CD4 mAb to separate CD4+ cells by FACS,in combination with in situ hybridization using Dig-labelled IL2,IL6 cDNA probes.Results:The patients with POF had significantly higher percentage of Th1 cells as compared with the controls(49. 76±9. 22%,20.06±7.10% respectively, P<0.001).The ratio of Th1/Th2 in the patients with POF was significantly higher than that of the controls (1.15±0.17,0.63±0.09,P<0.001).Conclusions:The findings of this study suggest that patients with POF have increased Th1 cell activation,which may be related to the pathogenesis of POF.
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