微量元素对乙酸底物产甲烷的影响

摘要

越来越多的研究成果表明,厌氧发酵工艺反应器中的甲烷生成是以乙酸降解途径为主进行的,其中Methanothrix常为消化器中的主力产甲烷菌.文章以乙酸作为碳源和能源底物对Methanothrix进行半连续培养试验,分析微量元素对Methanothrix产甲烷代谢的影响.结果表明,通过优化培养液的微量元素浓度,可使产甲烷速度提高1.42倍,产甲烷菌浓度提高1.61倍,对Methanothrix产甲烷有显著的促进效果;当乙酸负荷相对较低时,微量元素优化作用不大,与高乙酸负荷时相比较,优化效果明显减弱.试验结果表明,微量元素具有调节或减轻高乙酸负荷对产甲烷的抑制作用.%More and more research shows that the main pathway of methanogenesis was proceed by acetic acid degradation in the anaerobic fermentation reactor. Methanothrix often as the main methanogen. In this paper, acetate as carbon and energy substrate, Methanothrix was cultured in semi-continuous way, the effects of microelements on methane fermentation was analyzed. The results showed that by optimizing the culture medium concentration of microelements, methane production rate was increased 1.42 times and bacterial concentration was increased 1.61 times than before. The microelements had a remarkable effect on the production of methane by methanothrix ; The optimization effect of microelements was weakened when the acetic load is low. The microelements could adjuste or alleviate the inhibitory action of acetic load on the production of methane.