H9N2亚型禽流感重组病毒的拯救

摘要

为建立 H9N2亚型禽流感病毒反向遗传学操作系统并拯救出适合生产疫苗的禽流感 H9亚型重组病毒，利用反向遗传操作技术，采用 H1N1亚型禽流感病毒的6个基因片段（PB2、PB1、PA、M、NP 和NS）和 H9N2亚型禽流感病毒的2个基因片段（NA 和 HA）为基因供体，构建与 H9N2流感病毒具有相同基因组合方式的病毒。研究了该重组病毒在293T 和 MDCK 细胞上的生长特性，并对其安全性、稳定性进行了初步评价。结果成功拯救出可以作为疫苗候选株使用的 H9N2亚型重组病毒，且重组病毒其具有良好的遗传稳定性。H9N2亚型禽流感病毒反向遗传操作系统的成功建立为禽流感病毒致病机理、传播机制及病毒基因功能的研究奠定了基础；重组禽流感病毒株的拯救为 H9亚型禽流感疫苗的研制开辟了新的途径。%The study aimed to establish operating system of H9N2 subtype avian influenza virus in reverse genetics and to rescue recombinant avian influenza virus H9 subtype suitable for the production of vaccine. The viruses with a common way of gene combination with H9N2 subtype virus by operative technique of reverse genetics including 6 gene segments of H1N1 subtype(PB2,PB1,PA,M,NP and NS)and 2 gene segments of H9N2 subtype(NA and HA)were constructed,as donors of gene segments.The growth char-acteristic of the recombinant virus in 293T and MDCK cells were investigated and the security and stability of the recombinant virus were evaluated preliminarily.The recombinant virus of H9N2 subtype considered as the vaccine candidate strain were rescured.The results of study indicated that the recombinant virus had good genetic stability.The successful establishment of H9N2 subtype AIV reverse genetic operating system laid a foundation for the study on pathogenic mechanism,spread mechanism,and virus gene function of avi-an influenza virus.The rescus of recombinant strains of AIV opened up new avenues for developing H9 subtype avian influenza vaccine.