STUDY ON THE TRANSLOCATION OF MITOCHONDRIAL PRECURSOR PROTEIN ACROSS MODEL MEMBRANES——THE INTERACTION BETWEEN Candida krusei APOCYTOCHROME c AND NON-BILAYER LIPID PHOSPHATIDYLETHANOLAMINE（PE）

摘要

＜正＞ The import rate of Candida krusei apocytochrome c across three types of liposomes:50%PS-50%PC（a）,50%PS-12.5%PE-37.5%PC（b）and 50%PS-25%PE-25%PC（c）follows this order:c＞b＞a.It may indicate that the import rate increases with increasing content of non-bilayer lipid PE invesicles despite the same binding ability of apocytochrome c to these liposomes.The lipid fluidity ofliposomes（a）,（b）and（c）measured before or following insertion of apocytochrome c by means of a flu-orescent probe,9-（9-anthroyloxy）stearic acid,decreases in the order of a＞b＞c.Using N-（7-nitro-2-1,3-benzoxadiazo1-4-yl）dioleoyl ethanolamine（NBD-DOPE）,an induction of hexagonal phase transi-tion can be detected in the case of liposomes（b）or（c）.It is suggested that local formation of non-bilayer lipid structure could facilitate the translocation of apocytochrome c across the membranes.