A regular nanostructure has been widely confirmed to result ina marked improvement in material performance in biosensing applications.In the present study,a regular nanostructured Prussian blue（PB） film with two heterogeneous crystal layers was synthesized in-situ using a secondary growth method.A PB seed layer was first controlled to form uniform cube-like crystal nuclei through an ultrasonic reaction with a single reactant.Then,well-defined 100 nm PB nanocubes were further crystallized on this seed layer using a self-assembly approach.In order to accelerate the electron transfer rate during the enzyme reaction for glucose detection,the graphene was used as the main cross-linker to immobilize glucose oxidase on the PB film.The as-prepared biosensor exhibited high electrocatalysis and electron conductivity for the detection of trace glucose with a sensitivity of141.5 μA mM-1 cm-2,as well as excellent anti-interference ability in the presence of ascorbic acid and uric acid under a low operation potential of-0.05 V.
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