不同染色技术和双色荧光原位杂交技术对宽翅菘蓝(Isatis violascens Bunge)的细胞遗传学分析

摘要

为了解十字花科菘蓝属植物宽翅菘蓝(宽翅菘蓝,Isaris violascens Bunge)的染色体结构,本文利用45S rDNA和5S rDNA双色荧光原位杂交、银染技术和CMA3对宽翅菘蓝进行分子细胞遗传学研究.45S rDNA和5S rDNA荧光原位杂交结果显示宽翅菘蓝染色体上有1对45S rDNA信号和1对5SrDNA信号;银染结果显示其中期染色体有1对银染点;CMA3染色结果发现宽翅菘蓝中期染色体存在1对CMA3信号.%Objective In order to understand the chromosome structure ofI. violascens Bunge, 45S rDNA and 5S rDNA dual-fluorescence in sim hybridization (45S rDNA and 5S rDNA D-FISH), silver staining and CAM3 staining were used to study the chromosomes of I. violascens Bunge. In the silver stained metaphase chromosomes ofI. violascens Bunge, there were two positive regions; fluorochrome Chromomycin A3 (CMA) staining showed two positive regions; D-FISH with 45S rDNA and 5S rDNA probes revealed one 18S-5.8S-26S rDNA loci and one 5S rDNA loci with different intensity.