微栓塞球联合凝血酶粉栓塞法建立犬急性心肌梗死模型

摘要

目的 通过微栓塞球联合凝血酶粉制剂栓塞冠状动脉制作Beagle犬急性心肌梗死模型的可行性以及安全性.方法 选用体重10~12kg的30只Beagle犬经股动脉行左冠状动脉前降支造影,经微导管先把聚乙烯微球2ml(4×104/ml)悬浮制剂快速注入,随后注入凝血酶粉制剂2ml(1000u/ml)栓塞左前降支来建立心肌梗死模型.行心脏超声检查测量左心室舒张末内径(LVEDd)、左心室收缩末内径(LVEDs)、左心室射血分数(LVEF)等参数.结果 全部Bea-gle犬均行左冠状动脉造影成功,栓塞即刻成功率为83.33%,术中死亡率为16.67%,2周的存活率为70.00%.术后1小时心脏超声检查提示心脏前壁心尖部、室间隔前1/3收缩减弱,LVEF值明显下降.术后1周示左心室前壁出现节段性运动减弱及反向运动,LVEDd增大,LVEF值恢复正常范围.结论 微栓塞球联合凝血酶粉制剂行冠状动脉介入封堵法建立Beagle犬心肌梗死模型,具有建模成功率高、操作简单快速、动物创伤小、术后恢复快及成活率高,且具有可逆性等优点,是一种新型有效的犬急性心肌梗死造模方法.%Objective To investigate the feasibility and safety of embolization of coronary artery in acute myocardial infarction (AMI) model in Beagle dogs. Methods 30 Beagle dogs with body weight of 10~12kg via femoral artery of left coronary anterior descending angiography ,the micro catheter put polyethylene microspheres 2ml (4 × 104/ml ) suspension rapid injection ,then the injection of thrombin powder preparation of 2ml (1000u/ml) embolization of the left anterior descending artery to establish the model of myocar-dial infarction. Left ventricular end diastolic diameter (LVEDd) ,left ventricular end systolic diameter (LVEDs) and left ventricular ejection fraction (EF) were measured by echocardiography. Results All the Beagle dogs were successfully performed with left coronary angiography. The success rate was 83.33% ,the mortality rate was about 16.67% ,and the survival rate was 70% in the first 2 weeks. 1 hours after operation ,echocardiography showed that the 1/3 of the anterior wall of the heart was reduced and the EF value decreased significantly. At 1 week after operation ,the segmental motion of the left ventricular anterior wall appeared to be attenuated and reversed ,LVDd increased ,and the EF value returned to normal range. Conclusions Micro ball embolization combined with thrombin powder preparation of coronary artery interventional method establishment of canine model of myocardial infarction Beagle ,a model of high success rate ,simple operation ,fast animal trauma ,rapid postoperative recovery and high survival rate ,and has the ad-vantages of reversibility ,is a new type of effective canine acute myocardial infarction model methods .