The objective of this study was to investigate the root-invading fungi varieties of alfalfa in various growing seasons. An experiment was conducted to isolate and identify fungi varieties from 31 alfalfa （Medicago sativa） varieties in Henan Province, China. The results of this study showed that the dominant fungi species in three seasons were obviously different, and Alternaria alternata and Rhizoctonia solani were the most common fungal species in three growing seasons and dominant fungal species were different in each season. Root-invading fungi and their incidence of three growing seasons showed a significant difference among 31 alfalfa varieties. The five varieties with higher incidence of fungi were Alfaking, WL- 525HQ, Sitel, Siriver, Aohan, respectively, Vernal, Golden-queen Grandeur and THG-1 and the five varieties with lower incidence were Algonguin, when compared to other alfalfa varieties.%为探讨紫花苜蓿（Medicago sativa）品种不同季节根部入侵真菌及其表现规律，以31个紫花苜蓿品种为材料，分离和鉴定根部入侵真菌，结果表明，不同生长季节苜蓿根部入侵真菌区系中优势菌种类明显不同，眷、夏、秋3个季节均有的建群菌种为细交链孢（Alternaria alternata）和立枯丝核菌（Rhizoctonia solani），3个季节各具有其优势菌种；不同苜蓿品种在3个季节的入侵真菌种类及其分离率均存在显著差异（P〈0．05），春季带菌率最低，夏、秋季带菌率相对较高。平均带菌率较高的5个苜蓿品种依次为：苜蓿王、WL-525HQ、赛特、四季旺和敖汉；较低的5个苜蓿品种依次为：阿尔冈金、佛拿尔、金皇后、飞马和THG-1。
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