红花甙对过氧化氢诱导SH－SY5Y细胞凋亡保护作用的实验研究

摘要

目的：研究红花甙（carthamin）对过氧化氢（H2O2）诱导神经母细胞瘤 SH－SY5Y 细胞（SH－SY5Y cells）凋亡的影响，并探讨其机制。方法实验分为空白对照组、H2O2损伤组及红花甙高、中、低剂量组。用H2O2作用于SH－SY5Y细胞，使其发生凋亡。 MTT法检测SH－SY5Y细胞活性，比色法测丙二醛（MDA）的含量，流式细胞仪检测细胞凋亡。结果与损伤组比较，随着红花甙剂量的增加，SH－SY5Y细胞存活率提高（P＜0．01），MDA生成量减少（P＜0．01），凋亡率下降（P＜0．05）。结论红花甙对H2O2诱导SH－SY5Y细胞凋亡有保护作用，与红花甙能抗氧化、降低细胞凋亡有关。%Objective To research for effect of carthamin on H2O2 induced neuroblastoma SH-SY5Y cells apoptosis,and to explore its mechanism. Methods Experimental cell strains were divided into blank group,H2O2 injury group as well as carthamin high,medium and low dose group. By using H2O2 to make SH-SY5Y cells apoptosis was taken. MTT method was used to detect SH-SY5Y cell activity,colorimetric method to measure malondialdehyde (MDA) content and flow cytometry to detect cell apoptosis. Results Compared with injury group,with the increase of carthamin dose,SH-SY5Y cell survival rate increased,MDA concentration decreased and the rate of apoptosis decreased. Conclusion Carthamin could protect SH-SY5Y cells from H2O2 injury which would be associated with anti-oxidation and anti-apoptosis of carthamin.