腰椎间盘退行性改变患者ADAMTS-7表达及其机制研究

摘要

Objective To investigate the expression of new type metalloproteinase ( ADAMTS-7) in patients with degenerative lumbar disc and explore its mechanism .Methods The degenerative lumbar disc tissue samples from 40 patients with lumbar disc her-niation undergoing the surgical resection of the degenerative disc tissue in our hospital from March 2014 to February 2016 were collect-ed.The samples were divided into the rupture group and the non-rupture group based on whether the posterior outer fiber ruptured or not.At the same time,normal lumber disc tissue samples from 20 patients with lumbar vertebrae fractures were also collected as the con-trol group.Qualitative and quantitative detection of tumor necrosis factor α( TNF-α) and ADAMTS-7 were performed by using semi-quantitative RT-PCR and immunohistochemistry .Results The expression levels of TNF-αwas increased with the degree of degenera-tion of lumbar intervertebral disc .ADAMTS-7 protein was shown weakly positive expression in the nucleus pulposus of the lumbar inter -vertebral disc in the control group while it was positive and strongly positive expression in the nucleus pulposus of the rupture group and the non-rupture group .The expression levels of ADAMTS-7 protein,mRNA and TNF-αin the three groups ranged from high to low:the rupture group >the non-rupture group >the control group ( P<0.05 ) .Conclusion There are high expressions of ADAMTS-7 and TNF-αin the nucleus pulposus of patients with degenerative changes in lumbar intervertebral disc .The more serious degeneration is ,the higher expression level is .The mechanism may be related to the expressions of ADAMTS-7 and TNF-αas the positive feedback path-ways,which can accelerate the disease progression of the degenerative lesions in lumbar intervertebral disc .%目的 探讨新型金属蛋白酶(ADAMTS-7)在腰椎间盘退行性改变患者中表达情况,并探讨其作用机制.方法2014年3月至2016年2月我院手术切除腰椎间盘突出症患者退变的椎间盘组织40例(依据后外层纤维破裂与否分为破裂组及未破裂组)及同期腰椎骨折患者正常腰椎间盘组织20例(对照组),采用半定量RT-PCR及免疫组织化学技术对不同髓核组织中肿瘤坏死因子(TNF-α)、ADAMTS-7进行定性定量检测.结果TNF-α表达量随腰椎间盘退变程度加重而增加,AD-AMTS-7蛋白在对照组腰椎间盘髓核组织呈现弱阳性表达,在未破裂组及破裂组椎间盘髓核组织中分别呈阳性表达及强阳性表达;3组ADAMTS-7蛋白表达量、mRNA表达量及TNF-αmRNA表达量由高到低排序为破裂组>未破裂组>对照组(P<0.05).结论ADAMTS-7及TNF-α在腰椎间盘退行性改变患者髓核组织中存在高表达现象,并且退变程度越重其表达量越多,而作用机制可能与7 ADAMTS-7及TNF-α在腰椎间盘退行性病变中表达呈正反馈通路加速其病程进展有关.