A disulfide bond containing polypeptide,PolyK 6-R 8,was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK 6 HC and CR 8 C.The molar ratio between CHK 6 HC and CR 8 C within obtained PolyK 6-R 8 was 9:1,and PolyK 6-R 8 could combine DNA compactly when weight ratio reached 5.Through in vitro investigation,it was found that PolyK 6-R 8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells.Cellular uptake of DNA mediated by PolyK 6-R 8 /DNA complexes was promoted after incubation for 4 h.Moreover,by examining the histological sections of hindlimb ischemia rats through immunohistochemistry,PolyK 6 R 8 /VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation.The results indicated that polypeptide-based PolyK 6-R 8 is a promising gene vector for the limb ischemia treatment.
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机译:modulatory effects of antimicrobials on panton-Valentine Leukocidin gene expression in community-associated methicillin-resistant staphylococcus aureus in vitro and disease severity in vivo in a murine model.
机译:ETUDE DE L'EFFET DEs RaYONNEmENTs sUR L'EmBRYON ET sEs ORGaNEs in VIVO ET IN VITRO(Rapport sur lesrechercheseffectuéesaucours delapériodedu1-10-1965 au 30-9-1906)