In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, ratesof primary production and physiological status. This study describes a preliminary investigation on the joint application of the threekinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were usedto obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer wasused to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora-tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima (Bacillariophyceae) and Thalassiosira sp. The higher value ofminimum fluorescence yield in dark-adapted state (Fo), actual photochemical efficiency of PSII (ΦPSII), and electron transportrate (ETR) exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higherβ-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.
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