Mutation Breeding of β-carotene Producing Strain B. trispora by Low Energy Ion Implantation

摘要

Ion beam bioengineering technologyasa new mutation approach has been widelyused in the biological breeding field.In this paper the application of low energy nitrogen ionimplantation in the β-carotene producing strain.Blakeslea trispora(-)was investigated.Theeffects of different fermentation conditions on β-carotene production by a high yield strain wereexamined.Results showed that two β-carotene high yielding strains B.trispora(-)BH3-701 andBH3-728 were screened out and the averaged production of β-carotene was raised by 178.7% and164.6% respectively after five passages in the shaking flasks.Compared with the original strain,the highest yield strain BH3-701 was potent in accumulating β-carotene,especially in the laterstage,and greatly increased production efficiency.