Surface Characterization and in Vitro Blood Compatibility of Poly (Ethylene Terephthalate) Immobilized with Hirudin

摘要

Poly (ethylene terephthalate)(dacron,PET) films were exposed under argon plasmaglow discharge with different glows and induced polymerization of acrylic acid(AA) in order to introducecarboxylic acid group onto PET (PET-AA) assisted by ultraviolet radiation(UV).HirudinimmobilizedPET (PET-HRD) films were prepared by the grafting of PET-AA,followed by chemicalreaction with hirudin.The surface structure of the treated PET was determined by X-rayphotoelectron spectroscopy (XPS).The wettability,surface free energy,and interface free energyof the films were investigated by contact angle measurement.The blood compatibility of the filmswas assessed by platelet-adhesion test and fibrinogen conformational change measurements to evaluatethe viability of the materials in biomedical engineering.Measurement by scanning electronmicroscopy (SEM) revealed that the amounts of adhered,aggregated and morphologically changedplatelets were reduced on the hirudin-immobilized PET films.Enzyme-linked-immunoassay measurementsthat disclosed fibrinogen conformational changes showed results consistent with theplatelets’ behavior.