Study on the Effect of Mutated Bacillus megaterium in Two-Stage Fermentation of Vitamin C

摘要

Bacillus megaterium as a companion strain in two-stage fermentation of vitamin Ccould secrete some active substances to spur growth of Gluconobacter oxydans to produce 2-KLG.In the fermenting system where Gluconobacter oxydans was combined with GB82-a mutatedstrain of B. megaterium by ion implantation, the amount of 2-KLG harvested was larger thanthat produced by the original B. megaterium BP52[1] being substituted for GB82. In this paper,we studied the effect of the active substances secreted by GB82 to enhance the capability ofGluconobacter oxydans to produce 2-KLG. The supernate of GB82 sampled at different cultivationtimes all had much more activity to spur Gluconobacter oxydans to yield 2-KLG than that of theoriginal B. megaterium, which might be due to the genetic changes in the active componentscaused by ion implantation. Furthermore, the active substances of GB82's supernate would losea part of its activity in extreme environments, which is typical of some proteins.