Studies on Mutation Breeding of High-Yielding Xylanase Strains by Low-Energy Ion Beam Implantation

摘要

As a new mutagenetic method,low-energy ion implantation has been used widely in many research areas in recent years.In order to obtain some industrial strains with high xylanase yield,the wild type strain Aspergillus niger A3 was mutated by means of nitrogen ions implantation (10 keV,2.6 × 1014～1.56 × 1015 ions/cm2) and a mutant N212 was isolated subsequently.However,it was found that the initial screening means of the high-yielding xylanase strains such as transparent halos was unfit for first screening.Compared with that of the wild type strain,xylanase production of the mutant N212 was increased from 320 IU/ml to 610 IU/ml,and the optimum fermentation temperature was increased from 28 ℃ to 30 ℃.