Effect of Ar^+ Implantation and Maize Genome DNA on Autotetraploid Rice

摘要

The effect of Ar^+ beam implantation and maize genome DNA on autotetraploid riceis studied.Better mutation types and higher mutation rates were discovered in M2 of T3 with ionimplantation and immersion in maize genome DNA.In the five agronomic categories investigated,the mutation rate of the seed setting rate was 9.1%,and the total mutation rate was 14.8% in theT3.However,the total mutation rate was 2.1% with the treatment of only ion implantation and1.3% with the treatment of only immersion in maize genome DNA.Mutant FA36(4) was discoveredin M1 with ion beam implantation and immersion in maize genome DNA.Its RuBPCase activity,PEPCase activity and seed setting rate were 32%,153%,and 36.79%,respectively,higher thanits parent IR36(4).Rapid analysis of polymorphicDNA (RAPD) analysis of three M2 plants ofFA36(4) (FM1,FM2,FM3) and two controls (purple maize and IR36(4)) were also conductedwith 40 random primers.S_5-3 was RAPD fragment amplified with a template of purple maize,FM2 and FM3 genome DNA using primer S_5.There was no S_5-3 in the RAPD pattern of IR36(4)or FM1.