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哈氏虫道真菌(Ambrosiellahartigii)生物学特性研究

     

摘要

研究了哈氏虫道真菌(Ambrosiellahartigii )的生物学特性。结果表明,哈氏虫道真菌菌丝生长和产孢均以PDA为最佳培养基,菌丝生长以甘露醇为碳源、KNO3为氮源最佳,产孢以蔗糖为碳源、甘氨酸为氮源最佳。菌丝生长和产孢的温度范围分别为10~30℃和20~30℃,最适温均为25℃,最适pH为6。菌丝生长、产孢和分生孢子萌发需要黑暗条件。分生孢子萌发最适温度为24~28℃,致死温度为46℃10 min,适宜pH为5.0~6.0。分生孢子在水中不萌发。%The biological characteristics of Ambrosiella hartigii were studied. The results showed that A. hartigii could produce many spores and grow very well on the PDA and PSA media. The mannitol and KNO3 were the best carbon and nitrogen sources for mycelial growth,respectively,while sucrose and glycine was favorable for conidial formation. The mycelium could grow between 10-30 ℃ ,while the conidia could form between 20-30 ℃ . For mycelial growth and sporulation,the optimum temperatures and pH were 25 ℃ and 6.0,respectively. The myce-lial growth,sporulation and conidial germination all required darkness. For conidial germination,the optimum temperature was 24-28 ℃ ,at pH 5.0-6.0,and the lethal temperature was 46 ℃ (10 min). The conidia could not germinate in water.

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