牡丹皮、黄连、大黄提取物对松材线虫生理代谢的影响

摘要

对3种中草药黄连、牡丹皮、大黄的乙醇提取物毒杀松材线虫作用机制进行了初步研究。测定了其对线虫体内总糖和蛋白含量的影响，处理前后线虫体内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、谷胱甘肽过氧化物酶（GSH-Px）和乙酰胆碱酯酶（AChE）酶活性变化和丙二醛（MDA）含量变化，处理前后SOD、CAT、GSH-Px和AChE表达量变化；并用松枝水培试验测定3种提取物对松材线虫病害的防控效果。结果表明，提取物处理后线虫体内总糖及蛋白含量显著下降，线虫体内糖及蛋白质的代谢受到干扰；线虫体内SOD、CAT、GSH-Px酶活性提高，POD活性显著下降，MDA含量升高，表明线虫体内氧化与抗氧化作用失衡，细胞功能受到极大影响；AChE活性被抑制，表明线虫的神经系统受到破坏，SOD、CAT、GSH-Px和POD表达量与酶活性变化基本一致。松枝水培试验表明中药提取物能够有效控制松材线虫病害发生。综上，3种提取物引起的松材线虫体内众多生理指标的改变是造成线虫死亡的主要原因。%The objective of this study is to identify the poisoning mechanism of the ethanol extracts of three tradi-tional Chinese medicinal herbs,Cortex Moutan Radicis,Coptis Chinensis Franch and Radix et Rhizoma Rhei,a-gainst Bursaphelenchus xylophilus. The effects of these extracts were evaluated on the metabolism of total sugar and protein and activities of protective enzymes,superoxide dismutase (SOD ),catalase (CAT ),peroxidase (POD),glutathione peroxidase (GSH-Px),acetylcholinesterase (AChE),and the content of malondialdehyde (MDA)from B.xylophilus. The gene expression of SOD,CAT,GSH-Px and AChE was also analyzed. The con-trol effects of the extracts on B.xylophilus were measured in water-potted plants. The extracts of three TCM herbs interfered with the metabolism of total sugar and protein of B.xylophilus. The activities of SOD,CAT,and GSH-Px were improved;the activity of POD was inhibited;MDA content was increased. Oxidation and antioxi-dation were imbalance,which greatly affected cell functions. The activity of AChE was inhibited,and the nervous system was damaged. The level of SOD,CAT,GSH-Px and AChE was consistent with enzyme activity. The re-sults revealed that the extracts can effectively control the pine wood nematode disease. It is concluded that the changes of some physio-biochemical indexes of B.xylophilus in vivo caused by the extracts of three TCM herbs were the main causes of its death.