黄花杓兰种子无菌萌发的培养条件研究

摘要

授粉15周后的黄花杓兰（Cypripedium flavum P.F. Hunt etSummerh.）种子经0.5%NaClO溶液处理后，无菌条件下播于培养基表面，20周后种子最高萌发率达到90%。KT和BA促进兰花种子萌发的机理是作为一种萌发诱导物质直接起作用，而不仅仅是拮抗ABA的抑制作用而促进种子的萌发。培养基，激素和种子预处理是萌发率高低的关键，种皮是阻碍黄花杓兰种子萌发的主要原因之一。%After pollinating for 15 weeks, Cypripedium flavum seeds werecollected and bleached in 0.5% NaClO solution for 10 minutes, then sowed in media axenically. After 20 weeks, the seed germination rate reached 90%. Medium, exogenous cytokinin and bleaching is vital to the germination. The mechanism of KT and BA improving germination rate is to act as a direct germination inducer, in addition to be an inhibitor of ABA. Seed coat is one of the important factors in the gevmination of Cypripedium flavum seeds.