Cloning and Expression Analysis of TcREMORIN Gene Related to Anthracnose Resistance in Taxus chinesis

摘要

[Objective]The paper was to obtain a pathogen-inducible responsive gene TcREMORIN from Taxus chinesis.[Method]Total RNA was extracted by Trizol reagent,and cDNA was synthesized using TaKaRa reverse transcription kit and Invitrogen M-MLV Reverse Transcriptase kit.The TcREMORIN gene was amplified by qRT-PCR.[Result]The full length of TcREMORIN was 778 bp with a 501 bp open reading frame(ORF)which encoded 166 amino acids.Bioinformatics analysis indicated that TcREMORIN possessed a conserved domain of remorin family genes and located probably in cell plasma membrane.The results of real-time quantitative PCR assay revealed that the target gene was induced by anthracnose of T.chinesis and its expression level was up-regulated by PEG,NaCl,H2O2 and 4°Cstresses.[Conclusion]The TcREMORIN gene may play a critical role in plant-pathogen interaction.