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Analysis of 18S rDNA Sequence of the Pathogen of Melon Powdery Mildew from Lands Overlaid with Sands in Ningxia

机译:宁夏沙地甜瓜白粉病病原菌18S rDNA序列分析

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摘要

[Objective] The paper was to measure and analyze pathogen of 18S rDNA sequence of the pathogen of melon powdery mildew from lands overlaid with sands. [Method]The melon powdery mildew was isolated from infected plants of "Yujinxiang", a major melon variety cultivated in lands overlaid with sands in the middle arid area of Ningxia. Genome DNA was extracted from its conidia using Chelex-100 method. 18S rDNA sequence was amplified by PCR, which was analyzed by Blast after sequencing, and the phylogenetic tree was constructed. [Result] 18S rDNA sequence analysis showed that the pathogen of melon powdery mildew belonged to Podosphaera. [Conclusion] The study provided reference for biocontrol and disease-resistance breeding against melon powdery mildew.

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