[Objective] The paper was to establish an affordable indoor virus inoculation and identification technology. [Method] Taking Tobacco Mosaic Virus(TMV) and Cucumber Mosaic Virus(CMV) of sweet(hot) pepper as the sources of virus,an affordable indoor virus inoculation and identification technology was developed in the paper. [Result] The suitable inoculation concentration of CMV was five to ten times,and the best seedling age for inoculation was five to six leaves. Suitable inoculation concentration of TMV was 20 to 30 times,and the best seedling age for inoculation was three to six leaves. Single inoculation technology was mainly used for indoor virus inoculation and identification of sweet(hot) pepper,and complex inoculation technology could also be adopted with first inoculation of CMV and late inoculation of TMV. For mixed inoculation technology,CMV∶TMV should be 1∶1. Complex inoculation and mix inoculation should base on the technology of single inoculation. Disease resistant materials,AB91-W22-49176,AB91-W22-48123,AB91-DL-6428,HY031-2-8-1-6,BYT-4-1-3-6-8,JFG-2-1-2-6,JF8S-1-1-5-4-8 and T502-1-1-3-5,were identified by this method. [Conclusion] This research provided scientific basis for standardization of indoor inoculation of sweet(hot) pepper virus.
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