Evaluation of Biological Characteristics of Bacteria Contributing to Biofilm Formation

摘要

Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants.Biofilm formation was determined for a total of 18 bacterial isolates obtained from the biofilms of wastewater treatment systems and of little carpolite in soil.Among these isolates,seven showed strong biofilm-forming capacity.The phylogenetic affiliation of the isolates showing high biofilm formation capacity was determined through 16S rDNA sequencing and the isolates were grouped into 7 bacterial species including Pseudomonas sp.,Pseudomonas putida,Aeromonas caviae,Bacillus cereus,Pseudomonas plecoglossicida,Aeromonas hydrophila,and Comamonas testosteroni.The biofilm-forming capacity was closely related with flagella,exopolysaccharide,and extracel-lular protein.According to the coefficient of determination,the relative importance of the five biological characteristics to biofilm formation was,in order from greatest to least,exopolysaccharide > flagella > N-acyl-homoserine lactones(AHLs) signaling molecules > extracellular protein > swarming motility.